FastQCFastQC Report
Fri 3 Jun 2022
HLMCVAFX3_n01_dad3a_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMCVAFX3_n01_dad3a_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8287091
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT209810.2531768988659591Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC146800.17714298057062483Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC104990.1266910185974789Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)