FastQCFastQC Report
Fri 3 Jun 2022
HLMCVAFX3_n01_dad3a_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMCVAFX3_n01_dad3a_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7681429
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT638510.8312385625122617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC292240.38045004386553594Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC292220.3804240070434811Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA219800.28614467438285246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT150480.19590104914072626TruSeq Adapter, Index 8 (97% over 37bp)
CACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA138000.179654072178497Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG116550.1517295805246654Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTC105700.13760460455990675Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC93680.12195647450493911Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT85900.11182815072560065TruSeq Adapter, Index 8 (97% over 36bp)
ACACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAG81270.10580062641990182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)