FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_9-DAM-GR-EV-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_9-DAM-GR-EV-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100887
Sequences flagged as poor quality0
Sequence length70
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC143041.2993159152574243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC93530.8495876506853111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC67350.6117794106025415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG29390.2669665460669442Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG24560.22309283332440114Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC23860.21673432423127895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC17600.15987108576992914TruSeq Adapter, Index 5 (100% over 35bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCA14330.1301677647206298Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC13760.12499012160194461Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13400.12172003121119607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCA13290.120720836925134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGT12380.11245477510407517Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACGT11930.10836716211563947TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)