FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_5-DAM-GR-EV-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_5-DAM-GR-EV-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936121
Sequences flagged as poor quality0
Sequence length70
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC278781.4398893457588653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC136970.7074454540806075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC108390.5598307130597726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC43290.22359139743848652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG41920.21651539340774673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG40220.2077349504498944Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT24880.12850436517139166TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)