FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_17-2x_dam126-GR-bZIP1_y_bZIP11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_17-2x_dam126-GR-bZIP1_y_bZIP11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720877
Sequences flagged as poor quality0
Sequence length70
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC85021.1793967625544997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC43140.5984377362573643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC32090.44515222430456236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG17820.247198898008953Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG17670.24511809920416383Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC14190.19684356693305516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC8530.11832809203234393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)