FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_16-2x_dam126-GR-bZIP1_y_bZIP11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_16-2x_dam126-GR-bZIP1_y_bZIP11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728342
Sequences flagged as poor quality0
Sequence length70
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC140920.8153478883230286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC81160.4695829876262916Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC60040.347384950432264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG35370.20464699694852062Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG30990.17930479037135014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC26380.15263182865428254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCA17460.1010216727939262Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)