FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_11-dam126_GR-EV-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_11-dam126_GR-EV-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592468
Sequences flagged as poor quality0
Sequence length70
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC170601.0712931123262759Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC96550.6062916177907499Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC65950.4141370501636454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG34580.21714722054069535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG33860.21262593659652818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC30030.18857521783797226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC21390.13431981050796626TruSeq Adapter, Index 5 (100% over 35bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA18780.11793015621036028Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTGAA16380.10285920972980306TruSeq Adapter, Index 8 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)