FastQCFastQC Report
Mon 23 Dec 2024
HLM3CAFX7_n01_1-DAM-GR-EV-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLM3CAFX7_n01_1-DAM-GR-EV-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287001
Sequences flagged as poor quality0
Sequence length70
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC245371.0728897801094097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC118850.5196762047764736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGCCGCGACCCCGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCC94120.4115433268284535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAGAGCACACGTCTGAACTCCAG57740.25247037495829694Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAG52440.22929592072762542Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCC37470.16383901887231356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT31750.13882809845732466Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG29400.1285526329022156TruSeq Adapter, Index 2 (97% over 35bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCA26550.11609089808006207Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC23270.10174897168825024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)