FastQCFastQC Report
Tue 24 Dec 2024
HLLT2AFX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLLT2AFX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247827
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGGACCAAAACCTGCG369991.6459896602363082No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCT305881.3607808785996431No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGGACCAAAACCTGC238461.0608467644529584No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGGACCAAAACCTGCG220260.9798796793525479No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGGACCAAAACCTGCG214390.9537655700371959No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGGACCAAAACCTGC211530.9410421709499887No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGGACCAAAACCTGC179160.7970364267356873No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGGACCAAAACCT161110.7167366527762146No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGGACCAAAACCTGC159160.7080616079440277No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGGACCAAAACCT153150.681324674897134No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGGACCAAAACCTGCG139200.6192647387899514No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGCG104200.46355880590454696No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC97930.43566520021336164No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATACACCGGACCAAAACCT92490.4114640495020302No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGAGCGAGCAGCC74090.32960721621370326No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGGACCAAAACCTGCG71850.3196420365090374No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGCGGCGGCTGACAAG68790.30602888923391347No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGAGCGAGCAGCCGG63930.2844080082675402No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGAGCGAGCAGCCGGC60150.2675917675159165No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC57500.25580260402602156No Hit
TCGATCGTTACCATCTTCAGGAAAGGACGAATCTCCGGACCAAAACCTGC55500.24690512214685562No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG54840.24396895312673084No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCCGGACCAAAACCTGCG54520.24254535602606428No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGGACCAAAACCTGCG53970.24009854850929363No Hit
CGATCATGATCGTCTTGACGATAGGACGAAACTCAGGACCAAAACCTGCG52950.235560832750919No Hit
CGATCATGATCGTCTTGAGGATAGGACGAAACTCAGGACCAAAACCTGCG51350.2284428472475862No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGGACCAAAACCT49500.2202126765093577No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG48440.21549701111339975No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGC48270.21474072515367065No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGAGCGAGCAGCCGG48080.21389546437514984No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACTCCGGACCAAAACCTGC47070.20940223602617108No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACTCCGGACCAAAACCTGC44750.19908115704633852No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGGACCAAAACCTGCG44580.19832487108660943No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGCGGCGGCTGAC44050.19596703838863044No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACAGGACCAAAACCTGCG42510.18911597734167263No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATACACCGGACCAAAACCT42360.1884486662007352No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG41590.1850231356772563No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGCGACTCGGCGGAGT41480.18453377417390218No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATACACCGGACCAAAACCT40560.1804409325094858No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGCGGCGGCTGACAAG40500.18017400805311085No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGAGCGAGCAGCC40250.1790618228182151No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGAGCGAGCAGCC38530.17140998840213237No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGCGGCGGCTGACAA37630.16740612155650766No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGCGGCGGCTGACAAG37200.165493162952487No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGATTCACCGGACCAAAACCT36800.1637136665766538No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAAACTCCGGACCAAAACCTGC35760.1590869759994875No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGACCAAAACCTGC35140.15632875661694606No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGCG34990.1556614454760086No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGTGTGCAGCGAG33740.15010051930152987No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGAGCGAGCAGCCGGC33260.14796512365053005No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGCGGCGGCTGACAA32910.146408064321676No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACACCGGACCAAAACCTGC31230.13893417954317658No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGC30310.13484133787876024No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGTGTGCAGCGAGCA29660.1319496562680313No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCACCGGACCAAAACCTGC28390.1262997552747609No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGCGGCGGCTGACAA28280.1258103937714068No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGTGTGCAGCGAGCAG28080.12492064558349018No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACACCGGACCAAAACCTGC27870.12398640998617776No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGCGACTCGGCGG27630.12291871216067786No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCAAAACCT27550.1225628128855112No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGAGCGAGCAGCCGGC27130.12069434169088636No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG26870.11953766904659478No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCTG25480.11335391914057444No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGTGTGCAGCGAGCA25290.11250865836205366No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGCGACTCGGCGGAGT24810.11037326271105383No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAATCACCGGACCAAAACCT24760.11015082566407468No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCACCGGACCAAAACCTGC24670.10975043897951221No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGCGACTCGGCGGAG24480.10890517820099145No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGCGGCGGCTGAC24270.10797094260367901No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGCGGCGGCTGAC24230.1077929929660957No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATACACCGAGCGAGCAGCC24070.10708119441576243No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGCGACTCGGCGGAGT23800.10588003436207502No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGCGGCGGCTGACAA23340.10383361352986685No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCACCGGACCAAAACCTGC23090.1027214282949711No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACCGGACCAAAACCTGCG23040.10249899124799196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)