FastQCFastQC Report
Tue 24 Dec 2024
HLLT2AFX7_n01_Donor1_ky9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLLT2AFX7_n01_Donor1_ky9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21974831
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGGACCAAAACCTGCG5409252.4615661435576No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCT4142321.8850292864595866No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGGACCAAAACCTGC3327871.5144007250840748No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGGACCAAAACCTGCG3280651.4929125052201766No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGGACCAAAACCTGCG3215611.4633150079743502No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGGACCAAAACCTGC3092531.4073054759784047No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGGACCAAAACCTGC2550991.160868995989093No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGGACCAAAACCT2535091.15363344546313No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGGACCAAAACCT2365001.0762312574781576No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGGACCAAAACCTGC2314641.0533141301518996No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGGACCAAAACCTGCG1816690.8267139801894268No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGCG1484570.6755774367502531No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGCGGCGGCTGACAAG1448700.6592542167901086No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG1121310.5102701358658913No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGGACCAAAACCTGCG944740.4299191197420358No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC874660.39802808949930035No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGCGGCGGCTGACAAG868350.3951566225924559No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGCGGCGGCTGACAAG832430.37881064932877073No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATACACCGGACCAAAACCT801760.36485377293686583No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGCGGCGGCTGAC786090.35772288760719023No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGCGGCGGCTGACAA772030.3513246586515273No Hit
TCGATCGTTACCATCTTCAGGAAAGGACGAATCTCCGGACCAAAACCTGC768710.34981383929642057No Hit
CGATCATGATCGTCTTGAGGATAGGACGAAACTCAGGACCAAAACCTGCG757150.3445532755177958No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCCGGACCAAAACCTGCG741350.33736323159891424No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGTGTGCAGCGAG722740.3288944520210417No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGC722740.3288944520210417No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGCGGCGGCTGACAA694830.316193557984587No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGTGTGCAGCGAGCAG685600.3119932981509619No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACTCCGGACCAAAACCTGC678740.308871544905169No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG658920.29985213538161No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACTCCGGACCAAAACCTGC639100.2908327258580509No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGGACCAAAACCTGCG631810.28751529420180755No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGTGTGCAGCGAGCA631050.2871694439879879No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGGACCAAAACCTGCG604370.275028281218636No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGATTCACCGGACCAAAACCT592090.26944006986902425No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGCGGCGGCTGACAA591740.2692807967442389No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACAGGACCAAAACCTGCG577530.2628143078779537No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGAGCGAGCAGCC574930.26163113609383387No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGTGTGCAGCGAGCA573870.2611487660587697No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGCGGCGGCTGAC554300.25224312305291446No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGAGCGAGCAGCCGGC539610.2455582024726379No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGAGCGAGCAGCCGG536210.24401097783186593No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGACCAAAACCTGC524880.2388550792495287No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC522510.23777657266169644No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGCGGCGGCTGACAA512040.2330120309002604No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGTGTGCAGCGAG507270.23084136574247147No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAAACTCCGGACCAAAACCTGC506260.23038174901094804No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG486390.22133958618384825No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGTGTGCAGCGAGCA481910.21930089018659576No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGCGGCGGCTGAC475230.21626104883354963No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATACACCGGACCAAAACCT447840.20379678915391888No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGTGTGCAGCGAG438840.19970119451658128No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGCG434290.1976306438943717No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGC434190.19758513728729016No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACACCGGACCAAAACCTGC431760.19647932673520904No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGTGTGCAGCGAGCA425060.19343038406074659No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCACCGGACCAAAACCTGC418400.19039964402911677No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATACACCGGACCAAAACCT408540.18591269257087803No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGAGCGAGCAGCCGG407990.18566240623192962No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGAGCGAGCAGCC401630.18276818602154438No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACACCGGACCAAAACCTGC393140.17890467508032257No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCTG392340.17854062222367034No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGGACCAAAACCT386680.1759649482628558No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG366460.16676351231097067No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCACCGGACCAAAACCTGC363750.16553028325906124No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGAGCGAGCAGCC343460.15629699268221903No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGTGTGCAGCGAGCAG325460.14810580340754384No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCACCGGACCAAAACCTGC323510.14721842456945403No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGGTTTCCAGCTAGGA292230.1329839578743518No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGAGCGAGCAGCCGGC287340.13075868478806504No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAAACACCGGACCAAAACCTGC284380.12941168921845178No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGTGTGCAGCGAGCAG279800.12732748661411777No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACCGGACCAAAACCTGCG276520.12583486990184362No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGATTCACCGGACCAAAACCTG272420.12396909901150094No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGCGGCGGCTGACAAG266790.12140707703281087No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATTCTCCGGACCAAAACCTG259630.1181488039657734No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGTGTGCAGCGAGCAG256030.11651056611083836No Hit
ATCGATTCCTTGGTTCTAGTGGAAAGGACGATTCACCGGACCAAAACCTG242760.11047183935111947No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGAGCGAGCAGCCGGC235940.1073682887481592No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGGTTTCCAGCTA226830.10322263684303193No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGCGGCGGCTGACAAG222710.10134776463127293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)