FastQCFastQC Report
Tue 24 Dec 2024
HLLT2AFX7_n01_Donor1_GECKO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLLT2AFX7_n01_Donor1_GECKO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42363656
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGGACCAAAACCTGCG13049913.080449430521294No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCT11226492.6500285999867432No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGGACCAAAACCTGCG7768491.8337628839210667No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGGACCAAAACCTGCG7765701.8331043005353456No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGGACCAAAACCTGC7735211.825907093570961No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGGACCAAAACCTGC6377231.5053540232693796No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGGACCAAAACCTGC5677241.3401204088712269No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGGACCAAAACCT5663701.3369242730136417No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGGACCAAAACCTGCG4817301.1371303742056633No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGCG3761140.8878223352583167No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATACACCGGACCAAAACCT3618210.8540835096951973No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGCGACTCGGCGGAGT3093280.7301730521086282No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGGACCAAAACCTGCG2473580.5838920040328909No Hit
TCGATCGTTACCATCTTCAGGAAAGGACGAATCTCCGGACCAAAACCTGC1999920.472083901351668No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGCGACTCGGCGG1994040.47069591916240655No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGGACCAAAACCT1947230.459646353468643No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCCGGACCAAAACCTGCG1917170.4525506486031328No Hit
CGATCATGATCGTCTTGAGGATAGGACGAAACTCAGGACCAAAACCTGCG1837020.43363112947569965No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGGACCAAAACCTGCG1822010.43008799807079917No Hit
CGATCATGATCGTCTTGACGATAGGACGAAACTCAGGACCAAAACCTGCG1778200.4197465865552303No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGCGACTCGGCGGAGT1766220.41691869087030636No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGCGACTCGGCGGAGT1754540.414161610603202No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGGACCAAAACCTGC1694270.39993479316327185No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGGACCAAAACCTGCG1589550.3752154913164246No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGCGACTCGGCGGAG1575320.37185647999785476No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGCGGCGGCTGACAAG1533130.36189747173851095No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACAGGACCAAAACCTGCG1498030.3536120678536338No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACTCCGGACCAAAACCTGC1496080.35315176763780726No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGATTCACCGGACCAAAACCT1301220.3071547932501387No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC1257230.2967708924838781No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGACCAAAACCTGC1249740.2950028675523189No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGCGACTCGGCGG1209570.2855206831062928No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGCG1207240.28497068336122827No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACAGCGACTCGGCGGAGT1200110.2832876369310524No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGCGACTCGGCGGAG1182650.2791661796139597No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCAAAACCT1082850.2556082506193516No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGCGGCGGCTGAC1040860.24569645263855414No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGAGCGAGCAGCC1009390.23826791530929245No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGACCAAAACCTGC999020.2358200623666664No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGATTCACCGCGACTCGGCGG980610.23147435622647866No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATTCACCGGACCAAAACCTG948760.22395611936797902No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACACCGGACCAAAACCTGC916160.2162608439649307No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGCGGCGGCTGACAAG905860.2138295146197958No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG902260.21297972960596223No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGCGGCGGCTGACAAG870700.20552994765135474No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCACCGGACCAAAACCTGC870220.2054166429828436No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACCGGACCAAAACCTGCG785110.18532630894746194No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCACCGGACCAAAACCTGC777980.18364326251728602No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGAGCGAGCAGCCGGC762660.1800269551806388No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGAGCGAGCAGCCGGC747390.17642245041362814No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGCGGCGGCTGACAA741990.1751477728928778No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG738160.17424369605871598No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGTGTGCAGCGAG684110.16148511828157608No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGCGACTCGGCGGAGT668450.1577885534714001No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACACAGCGACTCGGCGGAGT659370.1556452068253977No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATTCTCCGGACCAAAACCTG636850.15032932946108335No Hit
ATCGATTCCTTGGTTCTAGTGGAAAGGACGATTCACCGGACCAAAACCTG594340.14029478475606544No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGCGACTCGGCGGAGT568170.13411731980828095No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCAGTGTGCAGCGAGCAG544430.1285134597448341No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATACACCGCGACTCGGCGG542520.12806260158471686No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGAGCGAGCAGCCGG528610.12477912671182108No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGCGACTCGGCGGAGT528590.12477440568396646No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCAGTGTGCAGCGAGCAG527210.12444865476199693No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAATCTCCGCGGCGGCTGACAA527210.12444865476199693No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCAGGTTTCCAGCTAGGA520120.1227750503875303No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGATTCACCGGTTTCCAGCTA516540.12192998640155137No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGATTCACCGCGGCGGCTGAC513060.1211085275548456No Hit
TCGATCGTTACCATCTTCAGGAAAGGACGAAACTCCGGACCAAAACCTGC509880.12035788412595928No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCTCCGTGTGCAGCGAGCA487450.11506325138699078No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGCGACTCGGCGGAG482380.11386647082584185No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGATACACCGGACCAAAACCT475050.11213621411711963No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGAGCGAGCAGCCGGC450620.10636947859268804No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCGACTCGGCGG447980.10574630291587676No Hit
TCGATCGTTACCATCTTGAGGAAAGGACGAATCTCCGGACCAAAACCTGC442570.10446926488119912No Hit
TCGATCGTTACCATCTTCTGGAAAGGACGAAACTCCGCGACTCGGCGGAG434710.10261390093432919No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGCGACTCGGCGGAGT430730.10167441639125764No Hit
TCGATCGTTACCATCTTCAGGAAAGGACGAATCTCCGCGACTCGGCGGAG423820.10004330126748268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)