FastQCFastQC Report
Fri 19 Feb 2021
HLLMGAFX2_n02_N1KO_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLLMGAFX2_n02_N1KO_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5377224
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCC1130102.10164203685768No Hit
CGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGC827301.5385261986482244No Hit
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCC608771.132126911581143No Hit
GTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCG506200.9413779303224117No Hit
CGAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGC460200.8558319311228246No Hit
GCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAG433560.8062896394124552No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG419510.7801609157438857No Hit
GGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAA378500.7038947977618192No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC368250.6848329175053894No Hit
CGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGC328290.6105194799398351No Hit
CGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAA318220.5917923448976647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305680.5684717616376034No Hit
CCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCC293930.5466203379290132No Hit
CCCGGCCGTGCCTGAGGTTTCTCCCCGAGCCGCCGCCTCTGCGGGCTCCC284470.5290276172240547No Hit
CTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGG269450.5010949887897547No Hit
GTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCT268510.4993468748930675No Hit
CTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGC259910.4833534924340143No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCG241950.44995335883347987No Hit
GCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGC241810.4496930014446116No Hit
CCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCG233730.43466666071564064No Hit
CCCGTGCCGAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGG233260.43379260376729706No Hit
GGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAG193210.3593117935946131No Hit
GGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCA192670.3583075579518354No Hit
GCCGAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGG191340.35583416275758645No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCC186200.34627532719485No Hit
CGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGC184890.3438391259132965No Hit
GTGCCGAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTC182520.33943164725888303No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTG179620.3340385299180395No Hit
GGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCT168980.31425136836404804No Hit
CGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCC167860.31216850925310163No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA160640.29874150677003597No Hit
CGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCGCG143720.2672754566296662No Hit
GGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGG138060.2567495793368474No Hit
CCCGCCCCGGCGACGGGGGGGGTGCCGCGCGCGGGTCGGGGGGCGGGGCG131930.24534964509568508No Hit
GACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAA120420.22394454833944058No Hit
AGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA117990.21942548794694067No Hit
GGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGG117460.2184398492605106No Hit
GCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGG117330.21819808882799005No Hit
CACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCG112920.20999683107863834No Hit
CGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGC109660.2039342233092763No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTG107630.20015904117068584No Hit
GCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCGCGCCGGTCTCC100640.18715976868361817No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT99730.18546744565597414No Hit
GGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCG96660.1797581800572191No Hit
GTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCGCGCCGG96620.17968379223182818No Hit
GGACCGGGTCGGAGGATGGACGAGAATCACGAGCGACGGTGGTGGTGGCG95960.17845639311287756No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTC95580.1777497087716636No Hit
GCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGG79380.1476226394883308No Hit
GACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCG78120.145279422988516No Hit
CCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTC76740.14271304301252838No Hit
CCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGA76420.14211794040940084No Hit
GACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGG74540.1386217126160264No Hit
CGTGCCGAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGT74280.13813819175098527No Hit
CGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCG73530.13674342002490505No Hit
GTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTG72910.1355904087313454No Hit
GCCCCGGCGACGGGGGGGGTGCCGCGCGCGGGTCGGGGGGCGGGGCGGAC72740.13527426047343385No Hit
GCTCTCCGCCCGCTCCCGTGCCGAGTCGTGACCGGTGCCGACGACCGCGT71950.1338051009219627No Hit
GTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTCCCCGAGCCGCCGCC68960.12824461097398956No Hit
CCGGCCGTGCCTGAGGTTTCTCCCCGAGCCGCCGCCTCTGCGGGCTCCCG65610.1220146305974979No Hit
CCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTCCCC63320.11775592759386629No Hit
GGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCGCGCCGGTCTC61560.11448286327666468No Hit
ACCGCGTTTGCGTGGCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGT60770.11301370372519352No Hit
GTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGC60640.11277194329267295No Hit
CGAGAATCACGAGCGACGGTGGTGGTGGCGTGTCGGGTTCGTGGCTGCGG59930.11145155939198366No Hit
CGCGCCCCCGCCCCGGCGACGGGGGGGGTGCCGCGCGCGGGTCGGGGGGC59280.1102427572293808No Hit
GGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGCGCGC58940.10961046071355778No Hit
CCCCGCCCCGGCGACGGGGGGGGTGCCGCGCGCGGGTCGGGGGGCGGGGC58910.10955466984451456No Hit
GAGTCGTGACCGGTGCCGACGACCGCGTTTGCGTGGCACGGGGTCGGGCC58820.10938729723738495No Hit
GCACGGGGTCGGGCCCGCCTGGCCCTGGGAAAGCGTCCCACGGTGGGGGC57830.10754619855895904No Hit
GCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGT56900.10581668161861958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54560.10146499383324928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)