FastQCFastQC Report
Fri 19 Feb 2021
HLLMGAFX2_n01_N1KO_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLLMGAFX2_n01_N1KO_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5377224
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC797991.4840185195930093No Hit
GTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGG686001.2757512054547104No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG661091.2294261871924994No Hit
GCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCC518160.9636198901143043No Hit
GTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGC466580.8676967892726805No Hit
CCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACC422460.785647017866468No Hit
GTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGA379420.7056057177458108No Hit
CCCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGAC360280.6700111432962436No Hit
GGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCG311110.5785699089344242No Hit
CGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGA287100.5339186167435093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279810.5203614355660096No Hit
GTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGAC262370.4879283436955574No Hit
CTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGC258760.4812148424540246No Hit
CACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGC245230.4560531605155374No Hit
CTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCA240020.44636414625836673No Hit
GGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCG232810.4329557407316489No Hit
CCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTC232100.43163535683095955No Hit
GGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC211580.39347440240540477No Hit
GTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCG211210.39278631502053846No Hit
GGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGG208270.387318809854304No Hit
CCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCG200980.37376162867680424No Hit
CTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCT196740.36587651918536407No Hit
GGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGC189340.3521147714880392No Hit
CGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCC189340.3521147714880392No Hit
GGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGG174420.3243681126172166No Hit
GTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGAC169040.3143629501021345No Hit
CACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCC158410.2945943855044908No Hit
GGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCG153030.28458922298940864No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA151510.28176248562455275No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT150970.280758249981775No Hit
GGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC150130.2791961056485651No Hit
GGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGC142630.26524838838776293No Hit
CGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCG141520.2631841262331642No Hit
TGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGC136800.2544063628370326No Hit
CACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGG135780.2525094732895636No Hit
GTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGG130740.24313660729030442No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG126420.23510272214808237No Hit
GTGGGGTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGG122440.22770113352168334No Hit
CGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGC122060.22699444918046932No Hit
CGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACC120780.2246140387679591No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC120500.22409332399022244No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC119990.22314487921648793No Hit
GTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCG114670.2132512984394922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111670.2076722115351713No Hit
GGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGG111310.2070027211066528No Hit
TGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGAC107990.20082853159920436No Hit
GGGGTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCC107330.19960113248025374No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG101050.18792224389387535No Hit
CTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCGTGGGACG99600.1852256852234536No Hit
CATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCGTGG99320.184704970445717No Hit
TGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCC93800.17443945054176654No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA91300.16979021145483247No Hit
GGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCG86740.1613099993602647No Hit
GCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCG79430.14771562427006946No Hit
GGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCG77440.14401482995686993No Hit
GCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGG73600.13687359871933918No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT72390.1346233670012631TruSeq Adapter, Index 14 (97% over 44bp)
CGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCC71420.13281946223553268No Hit
CCCGCCTGGGACGCCGGACGGCCCTCGGCCCCCACCGAGAACCGCCTCGC68760.12787267184703482No Hit
ACCCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGA68400.1272031814185163No Hit
GGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGA68360.12712879359312537No Hit
ACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCT67720.12593858838687025No Hit
CCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGG64940.12076863452219956No Hit
GCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC63760.11857419367316668No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAG63240.1176071519430844No Hit
GGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAG63200.11753276411769344No Hit
GGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGA62640.11649133456222022No Hit
GCGACGAGACGTGGGGTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCC60640.11277194329267295No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC60490.1124929889474569No Hit
GGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGG59500.110651890269031No Hit
CACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGT59180.11005678766590345No Hit
GCGACGAGACGTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGG59070.10985222114607836No Hit
CGGGAGAAGACGAGAGACCACGGGCGAGGCCGGGGCGACGGGGAAGGCGC57280.10652336595983354No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT56450.10497981858297145No Hit
TCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCG55620.10343627120610933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)