FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19115683
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3780741.9778210383589223No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3319051.7362968406621933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC16550.011.7983378
TAGACCG17900.011.7257145
TCCCCCC32850.011.452451145
TATACTG36100.011.4277835
ACTGTCC18500.010.9456448
TACACTG25450.010.8066265
GTCCAGG24500.010.6593421
TCTATAC35000.010.350063
CTATACT34550.010.2705464
TATACAG55700.010.2651735
CTGTACT23450.010.1909954
TGTACTG34450.010.0843135
GTACAGG33250.010.035991
CAGGACT19850.09.8502844
GTCCTGA29800.09.7372821
TCTGGAC27900.09.6081213
GGCCTAG12901.506669E-89.5598961
TCCAGGG22000.09.555482
CCTAGGA11502.8427348E-79.4551972
TACACCG28500.09.3961765
Produced by FastQC (version 0.11.5)