FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_Takahashi.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_Takahashi.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15724670
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT48250.024.3296434
GTATAGG89450.019.853851
CCTATAC88950.019.6357363
TATACTG135750.018.6823335
CTATACT55650.017.9693364
TAGACAG75500.017.8512575
TATAGGG73800.017.7754422
GTATAGA119200.017.3919681
TCTATAC111900.017.3572443
TACACTG76200.017.3069025
TATACAG222150.017.2875085
CCCCTAT74350.017.2564411
TACACCG111600.017.0113965
ATAGGGG77400.016.9478133
CCCTATA78100.016.7967682
CTATACA134250.016.6247254
ACAGTGG84000.016.5617758
CTATACC76750.016.5225734
GTATAAG116500.016.4884151
GTGTAGG45800.016.4598871
Produced by FastQC (version 0.11.5)