FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_PY79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_PY79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10902545
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG82750.017.7756525
CTACACC43000.017.3572224
TACACAG69100.016.8828325
TATACAG137500.016.8106865
GTACTAC13650.016.4619751
CTATACT35900.016.3494074
GTGTAAG43200.016.2757451
GTGTAGG33350.016.0838031
TACACCG84950.016.0358495
GTCCTAG12700.015.9811211
GTATAAG80150.015.82659151
CTTACAC47400.015.748463
CTAAGAC20700.015.4049943
TAGACAG59400.015.3702735
TACACTG59100.015.3256885
TAGGACT20550.015.1624044
GTATAGA66000.015.1561211
TATACCG70700.014.9634225
GTATTAG53250.014.7013441
TCTATAC63450.014.506123
Produced by FastQC (version 0.11.5)