FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_PB-434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_PB-434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40551452
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG231750.018.8541975
GTGTAAG107850.017.272821
TATACAG333650.016.6142525
TCTATAC200250.016.4307143
TACACCG296100.016.3718665
CTATACT128050.016.1847924
TATACCG417200.016.0660065
TACACTG132800.015.9874795
GTATAGA208550.015.95338251
TACACAG140150.015.8211775
GTATAAG245350.015.6581361
CTACACT76600.015.2306264
CTATACA173650.015.1479024
CTACACC163200.015.0522074
TTATACT257200.014.8195644
CTTACAC114050.014.7423193
CTATACC213200.014.6830814
TAGACTG81850.014.5189645
GTATAGG184700.014.4813741
TAGGACA114100.014.4800424
Produced by FastQC (version 0.11.5)