FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_AK-S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_AK-S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15316642
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG146700.017.4858915
TACACTG135800.017.28865
GTATAAG117050.017.091771
TCTATAC113550.016.5944653
CTATACT107450.016.4552754
GTATAGG91650.015.8969191
TATACCG135950.015.77715
CCTATAC84850.015.7160353
TAGACTG66300.015.5199195
CTACACT88300.015.42832854
GTGTAGG83100.015.00295451
GTCCTAG24900.014.8463661
ACACTGT97050.014.5597426
TACACAG105550.014.5543325
TATACAG204850.014.5385295
CTATACA127600.014.4246334
TCTAGAC34550.014.2638873
GTATAGA115050.014.1757241
GTGTTAG61600.014.1204941
CTAGTAC35750.013.9878233
Produced by FastQC (version 0.11.5)