FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_AK-55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_AK-55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14069546
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG29750.022.4088555
TACACCG63150.019.5071755
TAGGACT31150.018.6109164
TAGACTG23750.018.3065475
CTAGACT16650.018.2797764
TATACCG89900.017.652385
GTATAAG69600.017.4972021
GTATAGA61350.016.7780841
GTATAGG56550.016.5358181
CTATACC53600.016.3589864
ACTGTGC12850.016.3525668
CTATACT31550.016.3077774
TACCCTG44250.016.2121545
TGTACTG35100.016.1029835
GTGCTAG23500.015.7315281
TATACAG45750.015.680615
TCTACAC38700.015.5440123
ATACTGT24350.015.4747356
TACTCTG28100.015.214745
GTCCTAA25450.015.09581851
Produced by FastQC (version 0.11.5)