FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_AK-37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_AK-37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22937910
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT130400.018.1161524
TACACTG204400.017.5839845
TATACTG219650.017.0889365
CTATACT161450.016.606944
TATACAG315250.016.2280395
TATACCG210150.015.5511895
TAGACTG101950.015.4947915
GTATAAG177550.015.3913371
TCTACAC147400.015.389483
TACACCG194050.015.3477485
TCTATAC168050.015.0940793
GTACTAG51150.015.0215911
GTGTAGG125500.014.9015571
GTGTAAG100350.014.880091
CCTATAC134050.014.6514153
CTTACAC103900.014.5085793
GTATAGG135750.014.4171561
GTATAGA177200.014.0718231
CTACACC188750.013.9746324
TAGACAG120650.013.8739825
Produced by FastQC (version 0.11.5)