FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_AK-35A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_AK-35A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9300420
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG91050.017.9865425
TAGACTG40950.017.1647325
TACACTG82800.016.9781595
GTGCTAG27450.016.3728751
CTACACT53950.016.2524094
GTGTAAG42550.015.6734261
TACACCG82050.015.5436585
CTAGACT21000.015.5280554
TATACCG83850.015.4692465
ACACTGT55700.015.2212326
TATACAG128700.014.9769125
CCTATAC52700.014.8522763
ACTGTGC48250.014.5669268
CTATACC65600.014.4707584
GTATAGA75800.014.4405381
TCTACAC63950.014.2793983
TAGACAG50000.014.2028425
CTATACT68600.014.1548264
GTGTAGG49450.013.7796191
GTATTAG53200.013.7621221
Produced by FastQC (version 0.11.5)