FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n02_AK-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n02_AK-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22377708
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG51650.020.9042225
TATACTG125200.020.02585
TATACCG254100.018.1086945
CTACACT52500.017.8054264
TACACCG145050.017.6349835
GTCCTAG35500.017.5604131
CTATACT77350.017.1440224
CTACACC91300.017.0643464
CTAGGAC33800.016.510163
CCTACAC60600.016.1450143
TCTACAC84600.016.0194853
GTATAAG190250.016.0025541
TAGGACT56200.015.9884854
GTATAGA139650.015.8315551
GTGTAAG70900.015.6426331
TGTACTG133050.015.630945
TAGGACC50250.015.2859264
TTATACC189150.015.2858124
TATACAG161400.015.175095
CTATACC125650.015.1675434
Produced by FastQC (version 0.11.5)