FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19115683
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1186190.6205323660159043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG44300.018.0691055
TAGGACC12100.017.2839324
TACACTG33600.016.7406675
TATACAG65550.016.611995
TACACCG39700.016.5298275
TCTACAC28700.015.0767333
TACACAG31100.014.8401055
TCTATAC39850.014.8396413
CTATACT32300.014.7357194
ACCGTGC38900.014.6442298
CGTATAC53100.014.3962433
GTATAGG36200.014.350251
CTACACC35700.014.342344
CTAGCAC16150.014.2894443
GTTACAC23200.013.9882173
GTACAAG47950.013.8431451
GTATACG51800.013.5106911
CTAGGAC9155.638867E-1113.3987943
GTACACT20500.013.367794
CTACACT23850.013.3043664
Produced by FastQC (version 0.11.5)