FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_Takahashi.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_Takahashi.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15724670
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT44200.024.1125324
GTATAGG86650.021.2609581
TATAGGG73600.019.5047652
TAGAGGT70300.019.4919034
GTGTAGG38450.018.8634571
TCTATAC111300.018.0440393
TACACTG82150.017.8275455
GTATAAG112400.017.68081
CCTACAC37700.017.3081363
CCTATAC93950.017.2862323
TATACTG139700.017.2820635
CTATACT53100.017.2037754
TATACCG117900.017.0338865
TATACAG216800.016.9549475
GTATAGA123000.016.8646981
ATAGGGG72800.016.8307883
TACACCG111700.016.811035
TACACAG99700.016.7982715
CTATACC75400.016.7311444
TAGAGTG58750.016.5365415
Produced by FastQC (version 0.11.5)