FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_PY79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_PY79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10902545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG59800.020.004435
TATACTG81850.019.1328015
GTGTAGG29700.018.0720731
CTATACT36900.017.879634
CTACACT26350.017.609334
GTATAAG76500.017.2561021
GTGTAGA37250.016.7457621
CCTACAC27400.016.6699183
TACACCG91900.016.4093465
GTAGGAC21650.016.4089833
TATACAG137700.016.0586625
TACACAG66150.016.0017135
GTATTAG54850.016.0007921
CTACACC45350.015.8271024
GTGTATG86200.015.4825791
GTGTAGT23000.015.45257951
TAGACAG57150.015.3501495
GTATAGG38100.015.039551
GTCCTGG23200.015.0067271
TCTACAC39700.014.9749993
Produced by FastQC (version 0.11.5)