FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_PB-434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_PB-434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40551452
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG225950.019.9584085
CTATACT120400.017.2823494
TATACAG327500.017.134745
GTGTAAG101950.017.073831
TACACTG124900.016.8918975
GTGTAGA113450.016.8774321
TATACCG416250.016.4424155
TACACCG293200.016.295565
GTATAGG182950.016.2142871
GTATAGA209650.016.1904131
TCTATAC199950.016.0993713
CTTACAC104350.015.7717813
TACACAG127400.015.3084345
CTATACC212350.015.2616884
TAGACCT102650.015.2560514
TAGGACT80450.015.2302854
GTATAAG239550.015.22925951
GTCTAAG78300.015.1910931
TTATACT252750.015.00234
CTATACA167750.014.7380224
Produced by FastQC (version 0.11.5)