FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_AK-S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_AK-S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15316642
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG137200.018.072655
TATACTG147100.017.5463685
CTACACT90550.016.013694
CTAGGAC24800.015.7867743
GTGTAGG81250.015.7115591
GTATAGG87550.015.575131
GTATAAG121200.015.4399911
CTATACT107600.015.3628744
GTAGGAC57700.015.0784513
TACACAG105700.014.8845145
GTATAGA122900.014.8723181
ACACTGT95150.014.8585266
TACACCG133550.014.6035455
GTGTAGA101050.014.5710121
TATACAG206550.014.3915825
TCTACAC95800.014.3793543
TCTATAC116000.014.3129383
TAGGACT48300.014.2602244
TAGGACC35900.014.1368774
TAGACAG79150.014.0149125
Produced by FastQC (version 0.11.5)