FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_AK-55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_AK-55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14069546
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG34450.021.676535
TACACTG10850.021.3826735
TATACCG97000.020.1805885
CTATACC53450.018.7185924
CTACACT13200.018.6744044
TATACAG44800.018.286995
TAGACTG20100.018.034985
TACACCG61200.016.9405065
CTAGACT14150.016.9082744
TTACACT21600.016.7825534
GTATTAG23100.016.6413231
GTCCTAA24900.016.6034931
GTATAGG54750.016.5595991
GTGTAGA36550.016.4707981
TAGGAGT31900.016.3637774
GTGTAGG29400.016.2824781
CTAATAC24950.016.2726863
GTATAAG70200.016.221331
TAAGACA32650.016.2099424
TAAGGCT91300.015.9612554
Produced by FastQC (version 0.11.5)