FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_AK-37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_AK-37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22937910
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG207950.018.0602465
TATACTG218650.017.7401435
TACACCG205450.017.397775
GTATAGG132800.016.0025791
CTACACT131500.015.9890684
TACACAG163450.015.6582275
CTATACT158800.015.5687894
GTACTAG49150.015.4948081
TATACAG312350.015.4359355
TAGACTG95900.015.4228415
GTATAAG176150.015.3995971
TCTATAC169800.015.1153243
GTGTAGA151300.014.90877151
ACACTGT143750.014.6770226
GTGTAGG117550.014.6233251
TCTACAC147900.014.5592883
GTATAGA172400.014.5145061
TATACCG198000.014.2441725
CTTACAC102900.014.2327183
ACACCGT177200.014.1977196
Produced by FastQC (version 0.11.5)