FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_AK-35A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_AK-35A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9300420
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG85500.018.401255
TATACTG88250.017.3349045
TATACAG132850.016.6998885
GTGTAAG41950.016.5970461
TATACCG84250.016.092575
CTACACT54300.015.8891574
GTATAGG53050.015.8585731
TACACCG80900.015.414655
GTATAGA73550.015.3827311
TACCCTG62650.014.9287135
CCTACAC48850.014.8418873
GTATAAG72850.014.8336561
GTCCTAG13750.014.7688551
GTGTAGG47600.014.6270181
GTGTTAG34250.014.3992491
TAGACTG36850.014.3627995
GTCTAGA18750.014.3117241
TACACAG67100.014.154765
ACACTGT56750.014.0533726
GTAAGAC34150.013.7999143
Produced by FastQC (version 0.11.5)