FastQCFastQC Report
Tue 21 May 2019
HLL2YAFXY_n01_AK-23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLL2YAFXY_n01_AK-23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22377708
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG49450.021.1127555
TATACAG156650.018.9296075
TATACTG121950.018.6679485
GTGTAAG68800.018.6598551
TACACCG147550.018.4755485
TATACCG246050.018.062835
GTGTAGG58850.017.9940871
GTGTAGA77950.016.9347461
GTATAAG184100.016.9015661
CTAGGAC31450.016.8287533
TAGGACT55150.016.8272514
CTACACC83750.016.188464
TAGACTG66750.016.183925
CTATACT72600.015.7786264
CTTACAC72150.015.6760963
CTATACC124600.015.65247254
CTACACT47550.015.5524344
GTATTAG89550.015.5510261
GTATAGA135750.015.5480741
GTAGGAC67350.015.501533
Produced by FastQC (version 0.11.5)