FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_HU2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_HU2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA1334.21052631578947No Hit
TNCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA615.789473684210526No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATGTTA25.263157894736842No Hit
TNAAAACACTGGACATCATCAGGGTGGAGAATAAGTACTCGATCTTCCAA12.631578947368421No Hit
TGCGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.631578947368421No Hit
TNCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCATATTTTA12.631578947368421No Hit
TCAGAAACAGTCCCACAAATTAAAGCTGACAACGCACACTCCTACAGTTC12.631578947368421No Hit
ATGGACTCGCCTCACACTTTTGATCCACCTGCTTCCCTGTCTCTTATACA12.631578947368421No Hit
TCCGACTCGGTGCCACTTTTGCAAGTTGATAACGGACTAGCCTTATGTTA12.631578947368421No Hit
TCCACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAA12.631578947368421No Hit
GNGGCGTGCGAGCGGCTTGTGCATGTGTCTCACGGAGGAGGGTGGGGGGC12.631578947368421No Hit
TNGGTCTCGGGGCCGGGGGGGGGGGAGGGGGTGGGGGGGGGGGGGGGGGG12.631578947368421No Hit
TNGGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA12.631578947368421No Hit
ACGGGGGGGGGGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.631578947368421No Hit
GNTTGGTGGGTGGGGGGGGTTGTGCTGGGGGTGGGGGGGGGGGGGGGGCT12.631578947368421No Hit
CTCGCAAAACAACATAATAACACTAATTCACGAGTAGAGACAAATAAATA12.631578947368421No Hit
CAATAATACCTCCACCAAACAAAATAGAAAAACCGACACCACAAATAGCA12.631578947368421No Hit
TCCGCCTCGGGGCCCCTTTGTGGTGTTGATATCGGACTGGCGGTAGTGGG12.631578947368421No Hit
TCGGAGGGCGGGAGTGCGGGGCGGGCGGGGGGGGGGCGGGGGGGGTGGGG12.631578947368421No Hit
GNACACAGGCCCTTCGCTGGGAACTGCTCTTTCTACACTCTGGGTGGCAG12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)