FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_Gecko_v2_L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_Gecko_v2_L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTTGTATGTATGAGACAGTCTCTCACATAGACACCCAGGCAGGAGA11.1904761904761905No Hit
GACAGCTAGCCGCGGCGGCGAAGCGTAGAGCTGCGGGACCCGCCACCAGG11.1904761904761905No Hit
CNCTACATCTCAGTCCTCGACCAAACAATAAAAAAAAAAATAAAATAATG11.1904761904761905No Hit
GACACGAAACTACCGGGCAAAAAACCCCGGGCCAGGGCGGGACCACGAGC11.1904761904761905No Hit
CCTACAACTCAGGCCATACTTATAAATTTATCCCGACGCACACCAACCAT11.1904761904761905No Hit
CAAAAAACCATGCCGACGAAAAAAACTACAAAAGAAACAACAACACACTG11.1904761904761905No Hit
CNTACAGTGAGGGCTCTGGTACCAAAAAGACACCAGAGAACCCAGCTCCC11.1904761904761905No Hit
CAAAAAAAAACCACAATAAACCCAAGACAGACTCAAACAAATACGCAAGA11.1904761904761905No Hit
CCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGT11.1904761904761905No Hit
GTGAACATATAGGTGTAATACTTAAAATAAAAAAATAATTTGAGCTGGCC11.1904761904761905No Hit
ATTGACATCACGGGTAACACCGGCACAGAAAAAACTGGGCGGCTTGAGCG11.1904761904761905No Hit
GGGGTGGGGGCGGTGGTGGAGGTCGCAGTAGTGTGGGGGGGGGGGGGGGG11.1904761904761905No Hit
ATATTTATACACAAATTACGCTCCCCACGATCAACACTACAAAAGAAAGT11.1904761904761905No Hit
TCTATAATAGTCAAAAAAACTATACAATTAATTTAATTAAAATCTTATAT11.1904761904761905No Hit
CACTTAATACACAAAATAACCACACGACGATCAACTCCAACAAATCGATA11.1904761904761905No Hit
CNTCCCTCAAGACCAAAGATCAAAGAGTGCCCGCGGTCGCCTCCCACGAA11.1904761904761905No Hit
GNTGTAGTTTCCATTTGAGCATTCCTTAATGATGGGGTGGTTATAGGTGT11.1904761904761905No Hit
TCTCCCTCTGCAGACGNTAAGCTCCGGATACTCAGGCACATCACGGAACA11.1904761904761905No Hit
CCCGACCATCCTAAAGAACTAACCCAAAAAATCTCGTATGTAGCCTTAAA11.1904761904761905No Hit
ACGAATATGATGGGAATTACTATGAAGAAGATTAATACAAATGAATGGGC11.1904761904761905No Hit
ATCTCAGCCTCAATATGACTTTGGGTCCCTTTTCATTCAAAGGCCCAGGA11.1904761904761905No Hit
ATCTCGAAGTAAGAGGGTAGTACTCGATGGTCCCCCAGAGAATACACAAG11.1904761904761905No Hit
GCCCTCGGCCCGTCAGAGGCCTCCTTCGTGCTGTCACTCAGCCCTTGGGC11.1904761904761905No Hit
TACTAAAAGCACAAAATAATAAAACTAGAAAACTGACTAACAAAAATTTA11.1904761904761905No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTC11.1904761904761905No Hit
ATCTCACCCAGCATACCAAAAAATCTAACCAAGAGTGTAGAACTCCAACG11.1904761904761905No Hit
TNTATCGGAGATGTGTATAAGAGACAGGAGAAGAGAATGGAACTAAAGTG11.1904761904761905No Hit
ANACCTAAGCTCCGTTCCAACAAAAATCACTAAATTGGCCGATATATAAA11.1904761904761905No Hit
GACTACAACACGGTGGAAAAAAAAACATTAAAAAAAAAATAAAACAATCA11.1904761904761905No Hit
ACCGGGGGAGGGACCGGCGGAGGGGGCGGCATCTGGCTCCGCGCGGGCTG11.1904761904761905No Hit
ANTTACCATTTTAATTCTTTTTAAGTGTATGTTTCATTAGCGTTAAGTAC11.1904761904761905No Hit
CACAAAGTGCTGATTGGTGCATTTACAATCCTCTAGCTAGACATAAAAGT11.1904761904761905No Hit
GNTTAGTATTGTAATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAA11.1904761904761905No Hit
CAATAATACACATCTAACTATTCCGACGATCAACTCCAAAAAATACACTA11.1904761904761905No Hit
CCAAAAGATCATAAATAAAAAAGAGTCAATAACTCTCAGTTAGACGTATC11.1904761904761905No Hit
CTCACACTGTAATCTTCTTCAAGAGTATTTTCCCTTTACTTGGATATTCA11.1904761904761905No Hit
CAACAGACACAGCCTACCATCAACTCGAACTCAGCGAGAAAAACACGTAG11.1904761904761905No Hit
ATACACCTAAGTGTGAGACTCAGAAACACCAGAAGAGTGGAAGGGGGTCT11.1904761904761905No Hit
CCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACC11.1904761904761905No Hit
CTCCCTCCGCAAGCAGCGCGCCCCAGCACCGGAAGTGACGCGTTACGTGC11.1904761904761905No Hit
CNCTTACACATCTGACGCTGCCGACGATCATCTCGAACAAAGCGTGTAGA11.1904761904761905No Hit
ANCTAACAGACTAGATCACCCTAGTCCCCCTAACATAAAAATGTGCACCG11.1904761904761905No Hit
ACATAAGACCATTAATACCTTTTAAGGCTGTAGAAGTTCAGAATCGCCCT11.1904761904761905No Hit
GCTGCACGGGCGGGTGCTTGCAGATTCCTCCAGGGCCACCTGCAGGCCCC11.1904761904761905No Hit
ACAGAATATCTCCCACTTATACAAATAAATAACATACTAGACTTATATTA11.1904761904761905No Hit
AAACACATCCAACAGAGCCCCCGCTCAGCAACAACGACACAAAAACAAAA11.1904761904761905No Hit
AACTACTCGCTGCCACTTATTCAAGATGATAACGGACTAGCCTTATTTAA11.1904761904761905No Hit
CACACATGACTCTCCACACCAACATATCCAACAAAGCTATTAGATCACGC11.1904761904761905No Hit
ANGAACAACTTTTTAAAAATTTCATGTAGTCCCAATTTGTCTATTTTTTT11.1904761904761905No Hit
GATCCCAGCTCGGTCTCCGGCTCTGGCCTCTCCGCTCCGCCGCCGCCGCC11.1904761904761905No Hit
ANCTAAGCGTGTAGATCTCGGAGGTCGCCTTATCATTAAAAAATTATTTT11.1904761904761905Illumina RNA PCR Primer (95% over 22bp)
CCTACATCAACGCAGCCCACTAACAGCACCAAAAAACCTTTTAGATCTCG11.1904761904761905No Hit
AAAGAAATAAAAGAGAACATATCCTTGATCGCCTTATCATAAAATATTGG11.1904761904761905No Hit
CAGAACAAAACATTTTAAAACAAAACCACAACAAAACGCTCCTCATACTC11.1904761904761905No Hit
ATCTCGAACTAAGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA11.1904761904761905Illumina Single End PCR Primer 1 (96% over 33bp)
AAACACGCAGATGTGTAAAAGATACAGAAAAAGTACTAGGAAGCAGAAAA11.1904761904761905No Hit
AAATATAAGACAATTACTCGCAGCTAGCCTCTCACTTCAAAGGCTCGACT11.1904761904761905No Hit
ACGGCGCGTGGGTGTTTGGAGATGCCTCTCGGAAGCAGGGAGGGAGGGCT11.1904761904761905No Hit
TACAAATCTGAATCTACATACGATAAACTCAAACAAAACTGGAATAACTA11.1904761904761905No Hit
AAAATCAACCTCGTAACATGCACCACGCCATATCTTACAAACAAAAACCA11.1904761904761905No Hit
GCCTGTAGCTCGCTCGCGCCTCGGCGACAAAAGAAAAAGTGTCACAATGG11.1904761904761905No Hit
GTTATATTAGAAGCCACCCACAAAGCATAATGTTCATTGATCGATCAATG11.1904761904761905No Hit
AATCTGAAGCAGCCATCGAACGTAACTATGTAAAACTATAAATCTCCTAC11.1904761904761905No Hit
AAACACAAATGACACTCACAAAGAACAAAACCAACAAATAGAGCACAAAT11.1904761904761905No Hit
CAAATAGTCCTGAACATCTCGGAGCGCGCCATCTAAATAAAGTGCGGCCC11.1904761904761905No Hit
CATCTTACTCTGACCACGAACAACTAGTAGTAACCTTATACATACCCGTG11.1904761904761905No Hit
AAACACATCTAACCCAAACTACGAACATCACTAACTACACCTCTCTATCT11.1904761904761905No Hit
ACAACGCTGCCGACGAACAACTCGAACAAAGCGTGAATAGCACGTTGGTC11.1904761904761905No Hit
GNCAGAATACACCTCTGACGCTCCCAACGAACAACACGAACGACGCGAGG11.1904761904761905No Hit
GCAAAAGCACATGTAGAACAATGCGTGTAAAGCAACGCGATGCACCTACC11.1904761904761905No Hit
CAACAGACGAAGCATACCATCATCACGAACTAAGCCAGTAAATCACAATT11.1904761904761905No Hit
CAACTGACTCTGCCCACCATCATCTACAACTAAGCGAGAAGATATCCTAA11.1904761904761905No Hit
GGAGATGGATCCGTCGCCCAAAAGTTCGGAATGACGGTACCCCTTTCCCT11.1904761904761905No Hit
CNCAATAAAACTCTTTAACCCAAATCAAAAAAAAAAAATACATATCCTGT11.1904761904761905No Hit
GGGAAGCGAGGCGCACCCGGGGGACGCGCCGACCCGGTTCGGAAGAGCGG11.1904761904761905No Hit
CGAACATCTCAAAATATGCGAGAATATCACGCACATACCCCTAAAATAAT11.1904761904761905No Hit
CNTCCAACGAAGCCGTAGTGAGACTCGAACTTGTCTAGTAGATCACGATA11.1904761904761905No Hit
CCAAATAATCGTTAATATCTCCTTAGTCAAATTAACAAAAAATAACTCTT11.1904761904761905No Hit
GNCGACGTTAATCTCTAACAAACAGGGAACATATATCTGCACCCCCAAAA11.1904761904761905No Hit
GTGAACATATATGTGTAAAACATAAAATTAAAAATAGAAAGTCTTCATGA11.1904761904761905No Hit
CAACAACTAATCGTTTAGACATCCTCAGACACCCTCTAAATGAAAAAATG11.1904761904761905No Hit
CATAACGAACACGAAGTCTATTACACCTAGCGATCTCGAAGACTTGAGCC11.1904761904761905No Hit
CCCGCCCGTACACCCCAACAGACAGTCTCCCGCTCGCAGCACACCCTATG11.1904761904761905No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC11.1904761904761905No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)