FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_7C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_7C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464023
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC504302.046652973612665No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG481551.9543242899924231No Hit
GTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA393011.5949932285534671No Hit
CTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG332351.3488104615906589No Hit
GCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC321581.305101454004285No Hit
GTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA294061.193414184851359No Hit
GCTATCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC127610.5178928930452353No Hit
CTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG110160.44707374890575297No Hit
GTTGCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA99290.40295890095181747No Hit
GCTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC89780.36436348199671836No Hit
GTTCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA78270.31765125569038927No Hit
CATCTGACGCTGCCGACGATCATCTCGAACTAAGCGTGTAGATCTCGGTG73940.3000783677749761No Hit
GTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC69370.2815314629774154No Hit
GNTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC67080.27223771856025697No Hit
GTTGCTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAA65550.26602836093656596No Hit
GCTGTCTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG60880.24707561577144369No Hit
GTTGCTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA56210.22812287060632147No Hit
GCTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTAAGC55680.22597191665824548No Hit
TGCTATCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAG54500.22118300032101973No Hit
GTTGCTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTA52410.2127009366389843No Hit
CTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG51480.2089266212206623No Hit
GCTGTCTCTTATACAATCTGACGCTGCCGACGATCATCTCGAACTAAGCG51040.2071409236033917No Hit
GTTGCTGTCTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA45440.18441386301994744No Hit
GTTGCTGTCTCTTATACAATCTGACGCTGCCGACGATCATCTCGAACTAA43710.17739282466113343No Hit
GCTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG42240.17142697125797934No Hit
CNGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG40340.16371600427431077No Hit
CTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTAAGCG39140.1588459198635727No Hit
GNTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC37940.15397583545283466No Hit
TTTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCT36600.1485375745275105No Hit
CGATCATCTCGAACTAAGCGTGTAGATCTCGGTGGTCGCCGTATCATTAA35480.14399216241082166Illumina Single End PCR Primer 1 (96% over 33bp)
GCTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTAAGC34370.13948733433088895No Hit
GATCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCG33600.13636236350066538No Hit
GGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACT33520.13603769120661616No Hit
TTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC30360.12321313559167264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.11968232439388754No Hit
TTTTCGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC27730.11253953392480508No Hit
TGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAG27130.11010449171943607No Hit
CTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAAGCGT26940.1093333950210692No Hit
TTTTTCGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCT26430.10726360914650553No Hit
TTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG25840.10486915097789265No Hit
GGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACT25810.10474739886762421No Hit
GTTGCTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTA25400.10308345336062204No Hit
GCTGTCTCTTATACACATTGACGCTGCCGACGATCATCTCGAACTAAGCG25060.10170359611091292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)