FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_6C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_6C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466361
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG818903.3202763099157018No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC810203.285001668449996No Hit
GTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA769383.1194946725154997No Hit
GTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA723062.9316876158842926No Hit
GCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC438261.7769499274437117No Hit
CTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG393291.5946165220744248No Hit
GTTGCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA228270.9255336100432986No Hit
GCTATCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC179880.729333621477148No Hit
GTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC161910.6564732413462587No Hit
GTTCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA158880.6441879351806163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140040.5678000909031565No Hit
TTTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCT127870.5184561384160713No Hit
GCTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC108680.44064919936700264No Hit
CTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG105030.4258500681773674No Hit
GTTGCTGTCTTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA104260.4227280596798279No Hit
CNGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG97260.3943461642476507No Hit
GCTGTCTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG96050.38944015089437434No Hit
GTTGCTGTCTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA95930.38895360411553703No Hit
GTTGCTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAA89620.36336935266167447No Hit
GNTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA87270.3538411449094435No Hit
TTTTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATC86450.3505164085873885No Hit
TGCTATCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAG77240.31317394331162385No Hit
GTTGCTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTA74410.3016995484440437No Hit
TTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC67860.27514220343250645No Hit
TTTTTCGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCT64670.26220816822841425No Hit
GGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACT63680.2581941573030063No Hit
GCTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTAAGC63500.2574643371347503No Hit
CATCTGACGCTGCCGACGATCATCTCGAACTAAGCGTGTAGATCTCGGTG57910.2347993663539117No Hit
GCTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG57790.23431281957507435No Hit
CTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG56360.2285148037939296No Hit
GCTGTCTCTTATACAATCTGACGCTGCCGACGATCATCTCGAACTAAGCG54720.2218653311498195No Hit
CGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAAC52460.2127020334817166No Hit
GTTGCTATCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA48250.19563235065750717No Hit
GGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACT47480.19251034215996768No Hit
GTTGCTGTCTCTTATACAATCTGACGCTGCCGACGATCATCTCGAACTAA46770.18963160705184684No Hit
GTTGCTGTCTCTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA46470.18841524010475352No Hit
TGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAG46350.1879286933259162No Hit
CNGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG45570.1847661392634736No Hit
GTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA44450.18022503599432524No Hit
TTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG43580.17669757184775464No Hit
TTTTTTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCAT40210.16303371647540646No Hit
TTTTCGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC39350.159546797893739No Hit
TTTTTTCGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATC38290.1552489680140093No Hit
TTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAA36480.14791022076654634No Hit
GATCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCG36470.14786967520164324No Hit
GTTGTTGTCTCTTATATACATCTGACGCTGCCGACGATCATCTCGAACTA36190.14673439938435615No Hit
TTTCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCG35750.1449503945286193No Hit
CGGTTGTTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAAC34830.14122020255753315No Hit
GCATCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC32090.13011071777408093No Hit
CTGTCTTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCGT31950.12954307986543737No Hit
GCTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTAAGC31750.12873216856737515No Hit
GTTACTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA31010.12573179676454502No Hit
TTTTTTGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCT29990.12159614914442776No Hit
GTTGCTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTA29610.12005541767810958No Hit
GCTGTCTCTTATACCATCTGACGCTGCCGACGATCATCTCGAACTAAGCG29180.11831195838727583No Hit
CTGTCTCTTATACATATCTGACGCTGCCGACGATCATCTCGAACTAAGCG29090.11794704830314783No Hit
CTGTCTCTTATAACATCTGACGCTGCCGACGATCATCTCGAACTAAGCGT28950.11737941039450428No Hit
GTTGCTGTCTCTTATCACATCTGACGCTGCCGACGATCATCTCGAACTAA27900.11312212607967771No Hit
GNTGCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA27440.11125703009413462No Hit
TTTTTCGGTTGCTGTTTCTTATACACATCTGACGCTGCCGACGATCATCT27290.11064884662058798No Hit
CTGTCTCTTATACAATCTGACGCTGCCGACGATCATCTCGAACTAAGCGT26700.10825665829130447No Hit
GTTGCTGTCTCTTAACACATCTGACGCTGCCGACGATCATCTCGAACTAA25760.10444537519041211No Hit
GAGAAGCATCGTGTCAAAGCACCGACTCGGTGCCACATCGGTTGCTGTCT25360.10282355259428769No Hit
ATCGGTTGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGA25350.10278300702938459No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGC24690.1001069997457793No Hit
CGATCATCTCGAACTAAGCGTGTAGATCTCGGTGGTCGCCGTATCATTAA24680.1000664541808762Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)