FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_4A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_4A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551314
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4049276.180851658155905No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG1583412.416934984340546No Hit
CTGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG1431862.1856073453356073No Hit
CTCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC504410.7699371454337252No Hit
GNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG486350.7423701565823284No Hit
CTGAAACTAAAACACAAAAAAAGAAACCTAACAAAAAAACAAAAAAAACG303010.46251790098902296No Hit
CTGCTCGGAGATGTGTATAATAGACAGCTGTCTCTTATACACATCTGACG301210.45977036057194026No Hit
ACAGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA266820.40727707449223166No Hit
CNGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG186590.2848130924574826No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC157380.24022661713360097No Hit
GGGCTCGGAGATGTGTATCGGTTGCTGTCTCTTATACACATCTGACGCTG155600.23750960494337472No Hit
CTGCAACTAAAACACAAAAAAAGAAACCTAACAAAAAAACAAAAAAAACG149630.22839692922671695No Hit
CTGAAACTAAAACACAAAAAAAGAAACCTTACAAAAAAACAAAAAAAACG141600.21613984614384227No Hit
CGGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG95430.14566543444566998No Hit
GGGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG93970.1434368738851473No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT90310.13785020837041242No Hit
GCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGCT83100.12684478258865323No Hit
CTGCTCGGATATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG81070.123746167562721No Hit
GTGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG76870.11733523992286127No Hit

[FAIL]Adapter Content

Adapter graph