FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n02_2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n02_2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146371
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5109488.313002908545547No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG1754002.8537164450372425No Hit
CTGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG1707672.778338632666333No Hit
CTCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC673051.0950364044083898No Hit
GNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613260.997759490925621No Hit
CTGCTCGGAGATGTGTATAATAGACAGCTGTCTCTTATACACATCTGACG352250.5731024046547142No Hit
CTGAAACTAAAACACAAAAAAAGAAACCTAACAAAAAAACAAAAAAAACG335580.5459807095927011No Hit
ACAGCTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTA304790.4958861090552458No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC238150.38746440785953207No Hit
CNGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG218190.3549899607426887No Hit
CNGTCTCTTATACACATCTGACGCTGCCGACGATCATCTCGAACTAAGCG209110.34021701586188013No Hit
CTGCAACTAAAACACAAAAAAAGAAACCTAACAAAAAAACAAAAAAAACG196210.3192290214827579No Hit
GGGCTCGGAGATGTGTATCGGTTGCTGTCTCTTATACACATCTGACGCTG185360.3015763285359767No Hit
GGGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG146370.23814052226915688No Hit
CTGAAACTAAAACACAAAAAAAGAAACCTTACAAAAAAACAAAAAAAACG142420.23171396585074344No Hit
CTGCTCGGATATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG124250.20215180632604182No Hit
CGGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG121210.1972057983483262No Hit
GCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGCT114160.18573561537368963No Hit
GTGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACG105370.1714344936223342No Hit
CNCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC101130.1645361140744677No Hit
CGCTCGGAGATGTGTATAAGAGACAGCTGTCTCTTATACACATCTGACGC84500.137479498064793No Hit
CTGCTCGGATATGTGTATAATAGACAGCTGTCTCTTATACACATCTGACG68860.11203358860049288No Hit
CTGAAACTAAAACACAAAAAAATAAACCTAACAAAAAAACAAAAAAAACG68100.11079708660606397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)