FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120225564
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGACGCCGTGAGGC7080630.5889454592203036No Hit
CTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGA6421180.5340943960969898No Hit
CAACCGATCCGAGCCCACGAGACGACTACGTATCTCGTATGCCGTCTTCT3131790.2604928515868722Illumina PCR Primer Index 2 (95% over 23bp)
CAACCGAGCCCACGAGACGACTACGTATCTCGTATGCCGTCTTCTGCTTG2628730.21864983723428405Illumina PCR Primer Index 2 (96% over 28bp)
CAACCGATCCGAGCCCACGAGACGACTACGTATCGCGTATGCCGTCTTCT2112020.17567145702888945TruSeq Adapter, Index 2 (95% over 21bp)
CAACCGATCCGAGCCCACGAGACGACCTATTATCTCGTATGCCGTCTTCT2093220.174107729700482RNA PCR Primer, Index 44 (95% over 23bp)
CAACCGAGCCCACGAGACGACCTATTATCTCGTATGCCGTCTTCTGCTTG1982270.16487924315331137RNA PCR Primer, Index 35 (96% over 28bp)
CAACCGAGCCCACGAGACGACTACGTATCGCGTATGCCGTCTTCTGCTTG1700270.1414233332271995TruSeq Adapter, Index 2 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)