FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_7C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_7C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464023
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCT1448885.8801399175251206No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCGT1369365.557415657240212No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCGTA1006654.085392060057881No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCGT521142.1149964915100226No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCGTA386161.567193163375504No Hit
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCG380761.545277783527183No Hit
CAACCGAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGC187060.7591649915605496No Hit
CAACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATC167760.6808378006211793No Hit
CAACCGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCT164630.6681349971165043No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCG130470.5294999275574944No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTC128350.5208961117651906No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCGG120570.4893217311689055No Hit
CAACCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGAT120430.48875355465431936No Hit
CAACCGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTC117400.4764565915172058No Hit
CAACCGAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG111710.4533642746029562No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCGGA86900.35267527941094706No Hit
CACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTC81180.329461210386429No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTCGTT74600.302756914200882No Hit
CAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCTC63690.258479730099922No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCGG60540.24569575852173456No Hit
CAACCGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTC57090.2316942658408627No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCGGA55810.22649950913607542No Hit
CAACCGAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA49750.2019055828618483No Hit
CAACCGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCG48220.19569622523815727No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCG46990.19070438871715076No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGC45160.18327750999077524No Hit
CAACCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGC43330.17585063126439973No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGCGTT41320.1676932398764135No Hit
CAACCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCT39570.16059103344408718No Hit
CAACCGATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCT34180.13871623763252208No Hit
CAACCAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTC29490.11968232439388754No Hit
CACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTCGATCGC27740.11258011796156124No Hit
CAACCGAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTT26860.10900872272702No Hit
CAACCGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCTC25460.10332695758115895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)