FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_4C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_4C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4961849
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCT3739637.536767039867598No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGTA2990076.026120504674769No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGT2255754.546188326166314No Hit
CAACCGAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT1015592.046797474086777No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGTA998482.012314361037589No Hit
CTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGA927861.8699883853781119No Hit
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCG797931.6081303562442146No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGT724771.4606853211373423No Hit
CAACCGATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGTATGC674301.3589692068420463No Hit
CAACCGAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA630281.2702522789387585No Hit
CAACCGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATA484640.9767326655849463No Hit
CAACCGATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGTATGC339080.6833742824499496No Hit
CAACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATC301980.6086037684742119No Hit
CAACCGAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT287550.5795218677553469No Hit
CAACCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCAT273160.5505205821458896No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGTT263640.5313341861068324No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCT224930.45331891397743057No Hit
CAACCGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAT223090.4496106189446717No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCG216530.43638974100179184No Hit
CAACCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT216130.4355835899077138No Hit
CAACCGAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC211180.4256074701184982No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTC198850.40075786264354274No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGGA186760.3763919458250342No Hit
GCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGA166590.3357417769061493No Hit
TCCGCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCCCA163340.3291917992667653No Hit
CAACCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATA151690.3057126486517425No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGG144140.2904965467510196No Hit
CAACCGAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACT133820.26969784852380635No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGTT124040.24998745427359842No Hit
CACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTC122420.2467225423425824No Hit
CCGCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCCCAC122340.24656131212376675No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGGA105970.21356957859862322No Hit
CAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCTC105570.2127634275045452No Hit
TCTCCGCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCC102550.206676986744256No Hit
CTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG91370.18414506366477496No Hit
GCCTCACGGCGTCTAAGTCTCTGTCTCTTATACACATCTCCGAGCCCACG86370.17406817498879953No Hit
CAACCGATGTGGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT80970.16318513521874609No Hit
CAACCGATACACATCTCCGAGCCCACGAGACTCTATATCATCTCGTTTGC80310.16185498591351732No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT75620.15240286433545236No Hit
CAACCGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAT72510.14613503957899565No Hit
GCCTCACGGCGTCTAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA72470.14605442446958783No Hit
CAACCGATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA68770.13859752684936602No Hit
CTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT67440.13591707446155657No Hit
CAACCAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA66720.1344660024922161No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCG64600.13019340169360252No Hit
GCCTCACGGCGTCTAAGTCTCGTGCTGTCTCTTATACACATCTCCGAGCC63000.12696879731729038No Hit
CAACCGATGTGGCACCGAGTCGGTGCTTTGACACGATGCTTCTCCTGTCT60140.12120481699463244No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGCGG60030.12098312544376098No Hit
CAACCGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATC58450.11779882862215274No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCATCGC58170.11723452285629812No Hit
TCCGCCTCACGGCGTCTAAGTCTCTGTCTCTTATACACATCTCCGAGCCC57470.11582375844166157No Hit
CAACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATCAT56490.11384868826117038No Hit
GCCTCACGGCGTCTAAGTCTCGTGGCTGTCTCTTATACACATCTCCGAGC56300.11346576649148331No Hit
CAACCGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATATC54050.10893116658729438No Hit
CAACCAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAT53050.10691578885209929No Hit
CAACCCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT52890.10659332841446807No Hit
CAACCGAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC50600.10197811340087132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)