FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_4A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_4A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551314
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGA185930428.380627153575606No Hit
CAACCGATACACATCTCCGAGCCCACGAGACGACCTATTATCTCGTATGC1497792.286243645167977No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGACCTATTATCTCGTA1369152.0898860900271305No Hit
CTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG1257921.9201033563648453No Hit
CAACCGATACACATCTCCGAGCCCACGAGACGACCTATTATCGCGTATGC696671.0634049902050184No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGACCTATTATCGCGTA464610.7091859739893401No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACCTATTATCT271900.41503124411377623No Hit
CAACCGATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC210700.32161486993296307No Hit
CAACCGATCTCCGAGCCCACGAGACGACCTATTATCTCGTATGCCGTCTT147480.22511514483964593RNA PCR Primer, Index 35 (95% over 21bp)
CTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGC128700.19644913982141596No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT125760.19196149047351416No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCT121910.18608480680364275No Hit
CTGTCTCTTATACACATCTCCGAGCCCAGCTGTCTCTTATACACATCTCC106710.16288335439272184No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC106630.16276124148529594No Hit
CAACCGATCACATCTCCGAGCCCACGAGACGACCTATTATCTCGTATGCC105920.16167748943189106No Hit
CAACCGATCCACATCTCCGAGCCCACGAGACGACCTATTATCTCGTATGC98600.15050415840242126No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT98480.1503209890412824No Hit
CAACCGATCTCCGAGCCCACGAGACGACCTATTATCGCGTATGCCGTCTT91570.13977348666237033No Hit
CTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACCTATTA69730.10643666293509974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)