FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_2C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_2C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775720
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCT1155576.507613812988534No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGTA807554.547732750658888No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGT602383.392314103574888No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGTA299571.6870339918455612No Hit
CTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGA297741.6767283130223232No Hit
CAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCG267361.505642781519609No Hit
CAACCGAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGC237531.3376545851823485No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGT214471.2077917689725859No Hit
CAACCGATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGTATGC207421.168089563669948No Hit
CAACCGAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAG146380.824341675489379No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTGGTA140400.7906651949631699No Hit
CAACCGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCG123480.6953799022368391No Hit
CAACCGATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGTATGC115370.6497082873425991No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTGGT109550.616932849773613No Hit
CAACCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGAT92940.5233933277769017No Hit
CAACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATC91560.5156218322708535No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTC69290.3902079156623792No Hit
CAACCGAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTA64480.3631203117608632No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCG64410.3627261054670782No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGGA63530.3577703692023517No Hit
CAACCGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGA63170.3557430225486No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCT62370.35123780776248503No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGGGTA55010.3097898317302277No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGG50250.2829838037528439No Hit
CAACCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGC47410.2669902912621359No Hit
CAACCGATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTGGTATGC46420.26141508796431867No Hit
CAACCGAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCT41350.23286328925731536No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGGA39070.22002342711688777No Hit
CACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTC38540.21703872232108667No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGTT38230.21529295159146714No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGGGT37190.2094361723695177No Hit
CAACCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCG35440.19958101502489128No Hit
GCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGA31190.17564706147365577No Hit
CAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTC30680.1727749870475075No Hit
CTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG30400.17119816187236725No Hit
TCCGCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCCCA28530.16066722230982364No Hit
CAACCGATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGGGTATGC25490.1435474061225869No Hit
CAACCGAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACC25440.1432658301984547No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGG23900.13459329173518347No Hit
CAACCGATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAG23660.133241727299349No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCGTT21530.12124659293131801No Hit
AACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGC21390.12045818034374788No Hit
CAACCGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGA21290.11989502849548352No Hit
CACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGCGAGATCGCG20920.11781136665690536No Hit
CAACCGATGTGGCACCGAGTCGGTGCTTTGACACGATGCTTCTCCTGTCT20550.11572770481832723No Hit
CAACCGATGTGGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC20520.11555875926384791No Hit
CAACCGATACACATCTCCGAGCCCACGAGACCTAGCGAGATCTCGGATGC18740.10553465636474219No Hit
TCTCCGCCTCACGGCGTCTAAGTCCTGTCTCTTATACACATCTCCGAGCC17800.10024102899105715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)