FastQCFastQC Report
Thu 3 Nov 2022
HLJY3DRX2_n01_2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJY3DRX2_n01_2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146371
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGA218439335.53955659363875No Hit
CAACCGATACACATCTCCGAGCCCACGAGACGACTACGTATCTCGTATGC1785222.904510645387335No Hit
CTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG1661822.7037417689234835No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGACTACGTATCTCGTA1498952.4387561375647513No Hit
CAACCGATACACATCTCCGAGCCCACGAGACGACTACGTATCGCGTATGC1054201.7151584243775715No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGACTACGTATCGCGTA621381.0109705385503087No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACTACGTATCT323540.5263919148388537No Hit
CAACCGATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC246360.4008218833519812No Hit
CAACCGATCTCCGAGCCCACGAGACGACTACGTATCTCGTATGCCGTCTT204830.3332535572616752Illumina PCR Primer Index 2 (95% over 21bp)
CAACCGATCTCCGAGCCCACGAGACGACTACGTATCGCGTATGCCGTCTT181230.2948569163820407No Hit
CTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGC171240.2786034230605344No Hit
CTGTCTCTTATACACATCTCCGAGCCCAGCTGTCTCTTATACACATCTCC147760.24040201933791502No Hit
CAACCGATCACATCTCCGAGCCCACGAGACGACTACGTATCTCGTATGCC131060.21323151498664822No Hit
CTGTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCT123770.20137085769798146No Hit
CAACCGATCCACATCTCCGAGCCCACGAGACGACTACGTATCTCGTATGC122000.1984911096320089No Hit
CAACCGATCACATCTCCGAGCCCACGAGACGACTACGTATCGCGTATGCC89360.1453866029239042No Hit
CAACCGATCCACATCTCCGAGCCCACGAGACGACTACGTATCGCGTATGC82500.13422554544787485No Hit
CTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAG68870.11204985836357746No Hit
CTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACTACGTA65950.10729908754287693No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT65510.10658321796715492No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT65410.10642052033630901No Hit
ACAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCT64020.10415902326755089No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC62250.1012792752015783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)