FastQCFastQC Report
Tue 22 Nov 2022
HLJKYDRX2_n02_DO44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJKYDRX2_n02_DO44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24894675
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2031440.8160138664192242No Hit
ACACACCCAACACACCAAACACACCCAACACAAGATCGGAAGAGCGTCGT1782890.7161732378510666No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT586990.2357893806607236No Hit
ACACACCCAACACACAAACACACCCAACACAAGATCGGAAGAGCGTCGTG544190.21859694894590911No Hit
TGTGTTGGGTGTGTTTGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTT335570.13479589510608192No Hit
CACCCAACACACCAAACACACCCAACACAAGATCGGAAGAGCGTCGTGTA302650.12157218360954704Illumina Single End PCR Primer 1 (100% over 21bp)
TGTGTTGGGTGTGTTTGTGTGTTGGGTGTGTTTGTGTGTTGGGTGTGTTT257530.1034478256896304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)