FastQCFastQC Report
Tue 22 Nov 2022
HLJKYDRX2_n01_DO36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJKYDRX2_n01_DO36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11390501
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10064108.83552005306878TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC796990.6996970545896094TruSeq Adapter, Index 5 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC698420.6131600357174807TruSeq Adapter, Index 5 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC193660.1700188604522312TruSeq Adapter, Index 5 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC132060.11593871068533333TruSeq Adapter, Index 5 (97% over 42bp)
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122570.10760720709299793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)