FastQCFastQC Report
Wed 30 Nov 2022
HLJKYDRX2_n01_DO11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJKYDRX2_n01_DO11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47618600
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC32332676.789924525290537TruSeq Adapter, Index 10 (100% over 50bp)
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT4262100.8950494134644866No Hit
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT4218940.8859857282658457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC3564820.748619237020828TruSeq Adapter, Index 10 (98% over 50bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT2715460.5702519603684275No Hit
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT2449120.5143200346083253No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT2231980.46872020596993613No Hit
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2073350.4354075928313726No Hit
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGCCAAACACACCCAA1950710.4096529507377369No Hit
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGCCAAA1553570.32625276677600773No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1527780.32083681586606916TruSeq Adapter, Index 10 (98% over 50bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT1430050.30031332294523566No Hit
TGGGGTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGCCAAACACACCCAA1418550.2978983002440223No Hit
TGGGGTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT1383070.2904474302058439No Hit
TGGGTTGGGTGTGTTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTG1207960.25367398453545464No Hit
GGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT1123980.23603801875737634No Hit
TGGGGTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT1111170.23334789347019863No Hit
TGGGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT1087780.2284359472979046No Hit
TGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT1065930.22384740416559915No Hit
TGGGGGGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGCCAAACACACCCAA1008270.21173869034368922No Hit
GGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT980670.20594263586077707No Hit
TGGGGGGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT975370.20482962539847874No Hit
TGGGTTGGGTGTGTTGGGTGTGTTGGTGTGTTTGGCCAAACACACCCAAC856200.17980369015468745No Hit
GGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT824870.17322432830868612No Hit
GGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT796240.16721197179253483No Hit
TGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTTGGCCAAACACACCCAA792360.16639716413334285No Hit
TGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGTGTGTTGGGTGTGTT713230.14977970792925455No Hit
TGGGGTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT705590.14817529284775277No Hit
TGTGTTGGGTGTGTTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTG641630.13474356658952594No Hit
GGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT639760.13435086289811124No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC625860.13143183545925333TruSeq Adapter, Index 10 (98% over 50bp)
TGTGTTGGGTGTGTTTGGCCAAACACACCCAACACACCCAACACACCCAA570640.11983552645394865No Hit
TGTGTTGGGTGTGTTTGGCCAAACACACCAACACACCCAACACACCCAAC564930.11863641518230271No Hit
TGTGTTGGGTGTGTTTGGCCAAACACACCCAACAAGATCGGAAGAGCACA563670.11837181269503934No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGCCAAA562770.11818281091842263No Hit
TGTGTTGGGGGTGTTTGGCCAAACACACCCAACACACCCAACACACCCAA552090.11593998983590446No Hit
TGGGGGGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT538470.11307976294977172No Hit
TGGGGTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGCCAAA522640.10975543170105798No Hit
GGGGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT508080.10669780295934782No Hit
TGGGTGGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT493490.1036338741584171No Hit
TGGGTTGGGTGTGTTGGGTGTGCTGGGTGTGTTTGGCCAAACACACCCAA491160.10314456955895386No Hit
TGGGTTGGGTGTGTTGGGTGTGTCGGGTGTGTTTGGCCAAACACACCCAA489540.10280436636104379No Hit
TGGGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTTGGCCAAACACACCCAA480920.10099414934500385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)