FastQCFastQC Report
Tue 21 May 2019
HLJH5BCX2_l02.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJH5BCX2_l02.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151241724
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA21282821.407205593609869Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT2946200.040.80800230
GAGCGGT2964950.040.4912829
GCGGTTC2966200.040.26429731
TGCCGAG3126300.039.7997115
AGAGCGG3033200.039.65168828
AAGAGCG3040550.039.45034427
CGGAAGA3169700.038.27807224
TCGGAAG3195000.037.79335423
CGAGATC3285800.037.64711418
ATCGGAA3225150.037.5274522
GATCGGA3302400.036.8505121
CCGAGAT3394050.036.57005317
GCCGAGA3510150.035.71151416
AGATCGG3441050.035.55224220
GAAGAGC3437150.035.02712226
GAGATCG3562900.034.57864419
GGAAGAG3865350.031.31160525
ACCGATC87000.022.16148622
ATGCCGA5657550.021.97899814
CGGTTCA5751350.021.9569341
Produced by FastQC (version 0.11.5)