FastQCFastQC Report
Tue 21 May 2019
HLJH5BCX2_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJH5BCX2_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116435071
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA9019950.7746763859490411Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC1554700.040.1786231
AGCGGTT1564700.040.14236530
GAGCGGT1565650.039.86932829
TGCCGAG1710100.039.2018615
AAGAGCG1623750.038.47140527
AGAGCGG1634550.038.3471728
CGAGATC1784600.037.15461718
CGGAAGA1714400.037.1124224
TCGGAAG1710050.036.67585423
ATCGGAA1709350.036.61934722
GATCGGA1740400.036.0311321
CCGAGAT1847200.035.86869417
GCCGAGA1899050.035.03290616
AGATCGG1856900.034.19518720
GAGATCG1966550.033.2765619
GAAGAGC1904650.032.63260326
GGAAGAG2188400.028.23916625
GATCGGT72050.023.39409629
CGGTTCA3183750.022.3928451
ATCGGTT79300.021.79448930
Produced by FastQC (version 0.11.5)