FastQCFastQC Report
Fri 7 Oct 2022
HLH7LBGXM_n01_JK151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLH7LBGXM_n01_JK151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12964380
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT156093512.040182407488826TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTAT1820741.4044173342651172TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTTT745390.574952292357984TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGGAT568730.4386866167144129TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGGAT370920.2861070101308354TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTTT356030.27462169421136995TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTGGTAT321900.24829571487413976TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGGGGTT277520.21406345694896323TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGGGTAT221240.17065220241924411TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGGGTTT220530.17010454799998148TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAA213400.16460486347978076TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGGTT171450.13224697208813688TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTGGTTT168260.12978638392271746TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGGGGAT154250.11897985094543664TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTAGTAT149500.11531596574614444TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)