FastQCFastQC Report
Thu 18 Nov 2021
HLGLLBGXK_n02_910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGLLBGXK_n02_910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37877288
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCGTTGATTCAGCTAACGAGCGAGATTATCGCCAATTATTTACTT735710.19423513108963875No Hit
CTCCTACTCTCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCG709340.1872731754184724No Hit
GTCTACACCCCCGCAGGAACTATACAAGACTAGACCAAAGTGAAGCTCTA572970.1512700698107003No Hit
GTGGTAGTATAGCCAGTGAACGCGACATGAATCCTGCTCAGTGCTGTGTC554830.1464809201757No Hit
GTCTTAAGGGGGTGGTATGTCCCATCCAAGGCTAAATAACGTGAGGAGAC538140.142074585698955No Hit
GAGTAGGAGTATCCAACAGGATTAGAGACCCTGGTAGTTCCTACCTTAAA535820.1414620814457466No Hit
CCTTAAGACACTACTCTCAAAGTATCCGACTCTCCCCGGTCCATCGTAAG534560.14112942827374547No Hit
ACGCTAGACAGGTCAACCCACGCACCACCAACGGCCATGCACCACCAAAA520800.13749664442713005No Hit
CATCAGAGGCACGCCATTCGACTGAGTGACCTATCAGCTTGTACTTAGGG497150.1312527971907598No Hit
GCTCTGGGCTGCACGCGTGCTACAATGTTAGGATCAATAGGACTGCGAGC491260.12969777561688153No Hit
GTATTAGGGATCGTGAGCTGGGTTTAGACCGTCGTGAGCCAGGTTTGTTT488310.12891894477767257No Hit
GTCTTAGATGTGCGAAATTACAGCACGCGCTTATTTATAATTTGGCATTA477500.12606499177026612No Hit
GTCCTATTCCTTGTGATAGGTAAGCATAATTCCAAACAGGGTAGTGGTTT452540.11947529083919631No Hit
TACTACCACAGCGCGACACTACGTTTTTATTAAACAGTTGGGCTCTCCGC444750.11741864940277666No Hit
ATCCCAGACGGCGTAGCTGCGCGACATACCGTTTCCGATAATCGCTAACC441450.1165474149046785No Hit
GAATAGGACTAGATAGAAAGTTTGGCTGGGGCGGCACACCTTCGAAAGAG440840.11638636852775731No Hit
GGTCTAAGTCAGATGGAATGCTGCGCGATTCAGAGTAAAAGCCTCGTATG440810.11637844821413826No Hit
GTATGAAGTCTTTGTTTTCTTCCGAAAACAAGCTCAATGAGAGCCATCGG428770.11319976234834975No Hit
CCTCTAGATAACGTGATTTAATCACTTTCTGCTCGTAAGCATCCGGTGAT425820.11242093150914077No Hit
GTACTAATACCCAGGGAAACTGGTCATCAGGCGAACTGCGGCGTTCTGCT421980.11140713136589928No Hit
AGATAGATCTACCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAA408290.10779282825106168No Hit
GCCCAGAGCATCTAAAGGGCATCACGGACCTGTTATTGCTACCCTCTTCC401220.10592627434149984No Hit
ATCCAATAGCCATTGACATAACCGAAGCTATGTTTTGCCAGCAGAACGCC400860.10583123057807096No Hit
CCCTAATACGGGTGAACAATCCGACCCTTAGCAACTTGTACATCACTAGG397540.10495471587089339No Hit
CATTACACCTTCGCAAAAATGTCCACATCCCCCTACTCTCAGTTCAATAG394670.10419700586800196No Hit
CCTCTGATGAGGTCAACTTTTCCTCCATAATTCACATCTGGAGAAACAAT391390.10333105157898316No Hit
CACCAGGGGTGGAGCCTGTGGCTTAATTTGAATCAACACGGGGAAACTTA382650.10102360021129284No Hit
GATCTATCTCATGACGAGTGGTGGAATACTTTGACTCATGAGAGAGAAGC381120.10061966421672006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)